What immediate scientific protocol did the Montgolfiers establish with the successful 1783 animal flight?

Answer

The very first protocol for aerial biological safety testing.

The decision to send living subjects before humans established an unwritten, foundational protocol for aerial biological safety testing. By observing the outcome on the sheep, duck, and cockerel, the Montgolfiers moved beyond mere engineering feasibility to gather empirical data on biological survivability. This methodical approach, relying on observable life signs post-flight, ensured that the subsequent, more dangerous, manned flights would be built upon observed data rather than purely theoretical assumptions regarding the atmosphere's effect on living tissue.

What immediate scientific protocol did the Montgolfiers establish with the successful 1783 animal flight?
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